Chromatography Forum

Does anyone know what might be causing this? the baseline is steady then it drops, then it's steady, then it drops again.



presure is steady with no significant changes.

Lamp flicker?
Small air bubble accumulating in the cell gradually and then suddenly breaking loose?
Dirty cell windows?

If this were my problem, I'd check the noise with no flow. If it gets better, that eliminates the lamp. Setting a reference wavelength would diagnose lamp flicker (should get better with reference on).

I turned off the flow as you suggested and yes, it appeared to be the lamp.

The lamp has been replaced an that baseline behavior is gone. Now the baseline is drifting upwards slightly throughout the sequence. I can still integrate my peaks, however.

Thanks, Tom.