analysis of polyacrilic acid in ophthalmic solution

[William Jimenez]

have some method of analysis of polyacrylic acid in ophthalmic solution?
HPLC or GC method?

[Consumer Products Guy]

have some method of analysis of polyacrylic acid in ophthalmic solution?
HPLC or GC method?

Too big for GC.

[Meerkat]

have some method of analysis of polyacrylic acid in ophthalmic solution?
HPLC or GC method?

*just get you a simple gel column with H+ cation exchange capability and use USP as the mobile phase on an LC.

[William Jimenez]

Thank you.
I have some questions:
-such as the treatment of the sample?
-not be filtered with 0.45um filter pore size when diluted with water.
have you any application or description of the method?

[Vlad Orlovsky]

You can use Primesep C column in anion-exclusion mode similar to algininc acid, which is also an acidic polymer:

http://www.sielc.com/Compound-Alginic-Acid.html

This method is validated in two companies.

[MestizoJoe]

Vlad's suggestion looks fantastic based on the link provided.

If you have an SEC column handy you may want to try that too since your large polymer molecule will elute first, followed by smaller molecules. if you have other UV active large molecules in your solution you will need to make sure they don't co-elute.

[Vlad Orlovsky]

here is an article on analysis of aginic acid in Oral suspension using Primesep C column. I know at least two other places which use similar method

http://sphinxsai.com/s_v2_n2/PT_V.2No.2 ... -1638).pdf

Acrylic acid has pKa close to alginic acid. Algininc acid is a polymer with the wide range of MW, but it still elutes as a relatively narrow peak.