SEC-ICP-MS

[semamedo]

Could you please help me how to prepare mobile phase from tris buffered HN4NO3 at pH 7. I am a new user of HPLC and I found this forum is so helpful. Also, Did anybody have a method to determine the peaks concentrations and how can I integrated the peaks? I need to use SEC to characterise humic acid-bound metals in soil solution. any help? thanks in advance for your help.

[Gerhard Kratz]

For the buffer solution my recommendation is to purchase Standard buffers for example from E.Merck.
When you are running a SEC-ICP-MS System I guess for Instrument controll you have a Computer and Software. The Software should do the Peak Integration and will calculate, when you run a Standard sample before and calibration curves, the concentration.
To invest some Money and buy a book about High Performance Liquid chromatography I think is also a good idea. In Addition please Review carefully the Manual of your Instrument, or contact the manufacturer of the Instrument for free literature and publications.
Good luck