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- Posts: 2
- Joined: Thu Jul 18, 2013 12:00 pm
Dear forum members,
we made an analysis on an Agilent 1260 Infinity Quaternery HPLC, where we used the EZChrom Elite software to collect data and evaluate results.
The data aqusiton was made in scan mode, where the DAD scanned the spectra. When we integrated and evaluated the results on AAA nm, we realised that there is quite big difference in the heights and areas of an impurity peak between the mixed-view mode and the chromatographic view mode. Since we used to integrate in the below mentioned function it had an effect on the results, and this caused inaccurate purity of the samples.
Later we realised that this effect might be in relation with the timetable function of the DAD in the method-s instrument setup function. When we made timetable programming, for example because we had to control IMP-1 on AAA nm and IMP-2 on BBB nm, we programmed the method to collect data until XX minutes on AAA nm and from XX to YY minutes on BBB nm, and we set in the Signal function the data aquisiton channel for AAA nm.
In this case the detector collected the data as it was given in the timetable, but the chromatogram view visualised only the AAA nm in their legend, which AAA nm was ticked earlier in the Signal function.
It was very falsifying because we thought that the IMP-2 is so big, that we can already detect it on AAA nm, but it was not true, because the results were actually related to the BBB nm.
I think it should be a bug of the software, and its logical hierarchy, and if somebody has experiences on this issue or a useful link in relation with Agilent's technical support, please share it with me.
Thank you, watchmoretv
we made an analysis on an Agilent 1260 Infinity Quaternery HPLC, where we used the EZChrom Elite software to collect data and evaluate results.
The data aqusiton was made in scan mode, where the DAD scanned the spectra. When we integrated and evaluated the results on AAA nm, we realised that there is quite big difference in the heights and areas of an impurity peak between the mixed-view mode and the chromatographic view mode. Since we used to integrate in the below mentioned function it had an effect on the results, and this caused inaccurate purity of the samples.
Later we realised that this effect might be in relation with the timetable function of the DAD in the method-s instrument setup function. When we made timetable programming, for example because we had to control IMP-1 on AAA nm and IMP-2 on BBB nm, we programmed the method to collect data until XX minutes on AAA nm and from XX to YY minutes on BBB nm, and we set in the Signal function the data aquisiton channel for AAA nm.
In this case the detector collected the data as it was given in the timetable, but the chromatogram view visualised only the AAA nm in their legend, which AAA nm was ticked earlier in the Signal function.
It was very falsifying because we thought that the IMP-2 is so big, that we can already detect it on AAA nm, but it was not true, because the results were actually related to the BBB nm.
I think it should be a bug of the software, and its logical hierarchy, and if somebody has experiences on this issue or a useful link in relation with Agilent's technical support, please share it with me.
Thank you, watchmoretv
