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Vitamin B-12 Extraction from oil based formula
Posted: Thu Apr 25, 2013 8:47 am
by rojitea
Can any one help me with the analysis of Vit B-12 in soft gelatin capsules with a claim of 2 mcg/ cap and in an oil based formula.
I feel HPLC condition is not a problem but the sample extraction is the real problem.
Re: Vitamin B-12 Extraction from oil based formula
Posted: Thu Apr 25, 2013 12:19 pm
by Gerhard Kratz
After you opened the cap and added your extraction solvent, cool the sample down and than filter it.
Re: Vitamin B-12 Extraction from oil based formula
Posted: Thu Apr 25, 2013 1:50 pm
by rojitea
I require help in selecting suitable solvent for sample extraction.
Vit B-12 has aqueous solubility so can DMSO help to dissolve the oil in the cap ???
Re: Vitamin B-12 Extraction from oil based formula
Posted: Sun May 12, 2013 4:25 am
by kristind858
Yes the vitamin B12 will be soluble in the DMSO. You can use hexane to dissolve the oil, leaving the fatty acids/ triglycerides in this layer.