Vitamin B-12 Extraction from oil based formula

[rojitea]

Can any one help me with the analysis of Vit B-12 in soft gelatin capsules with a claim of 2 mcg/ cap and in an oil based formula.

I feel HPLC condition is not a problem but the sample extraction is the real problem.

[Gerhard Kratz]

After you opened the cap and added your extraction solvent, cool the sample down and than filter it.

[rojitea]

I require help in selecting suitable solvent for sample extraction.
Vit B-12 has aqueous solubility so can DMSO help to dissolve the oil in the cap ???

[kristind858]

Yes the vitamin B12 will be soluble in the DMSO. You can use hexane to dissolve the oil, leaving the fatty acids/ triglycerides in this layer.