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avoiding keto enol tautomerism in a compound in HPLC analysi
Posted: Thu Apr 25, 2013 1:48 am
by saipraveen7
We have been working hard on a compound which exhibits keto-enol tautomerism but we are unable to avoid it. can anyone suggest some tips to avoid this keto enol tautomerism while analysing by HPLC
Re: avoiding keto enol tautomerism in a compound in HPLC ana
Posted: Thu Apr 25, 2013 1:32 pm
by tom jupille
Increase the temperature to speed things up so that you will be looking at the "average" configuration.
Re: avoiding keto enol tautomerism in a compound in HPLC ana
Posted: Sat Apr 27, 2013 5:02 pm
by juddc
I don't think you'll ever entirely be rid of it, but pH can play a role too. Assaying for avobenzone works much better at pH ~2.2 than at ~3.0 for this reason.