avoiding keto enol tautomerism in a compound in HPLC analysi

[saipraveen7]

We have been working hard on a compound which exhibits keto-enol tautomerism but we are unable to avoid it. can anyone suggest some tips to avoid this keto enol tautomerism while analysing by HPLC

[tom jupille]

Increase the temperature to speed things up so that you will be looking at the "average" configuration.

[juddc]

I don't think you'll ever entirely be rid of it, but pH can play a role too. Assaying for avobenzone works much better at pH ~2.2 than at ~3.0 for this reason.