standard verification empower 2

[nattasha]

Hello

I have a similar question to the one posted orginally. I am struggling with how to get Empower to calculate the std verification: % difference = ((Average Area of check std (n=2) x Wt std 1 X 100) / (Average Area of working std (n=6) x Wt std 2) - 100).

This is what I'm struggling with: I know I have to set up a custom field, but our calibration curve is calibrated off the bracketing standards not the working std injections (n=6), so I have the bracketing stds set as sample type: standard, labeled "s."

What do I label my working standard and check std injections in alter sample? I currenlty have them as unknowns. Where do I enter my weight for std 2? I currenlty have the wt for std 1 entered in the components window in alter sample. Does anyone have suggestions?

This is my sample set:

Working Std (labeled as unk)
Check Std (labeled as unk)
Bracketing Std (labeled as std)
Sample (labeled as unk)
Bracketing Std (labeled as std)

Please help! How do I label in alter sample and what type of custom field would I use? Thank you.

[ydna6969]

The calibration curve will be generated using only injections named as function 'inject standards' remembering having processing as normal.
So dont worry about labels for calibration purposes

My way of doing std verif exactly how you require it, it maybe be the best way as it requires 5 custom fields bu tif you label samples correctly this will work for any sample set with verification as you have detialed :

Label all 6 S1 'A' and S2 'B' (N=2)
now create custom fields for average area and std weight :
A.%..AVE(AREA), B.%..AVE(AREA),A..(Value),B..(Value)

Enter weights for standard 1 and standard 2 into alter sample table
Then create a final custom field as you have detailed

I f you want to keep label 's' then in reprot order by 'A' or 's' and 'B' or 's'
Hope this helps

[ydna6969]

Apologies,, to read :

it MAY NOT be the best way as it requires 5 custom fields but if you label samples correctly this will work for any sample set with verification as you have detialed :

[nattasha]

Thank you for the reply. It makes sense. I understand how to create basic custom fields such as the entry for the std wts. Those will show up in alter sample for the analyst to enter correct? Can you walk me through the process of creating this that you mention: (I'm a beginner with custom fields, but trying to make the process more efficient where I work).

A.%..AVE(AREA), B.%..AVE(AREA),A..(Value),B..(Value)

How do I type it in to let empower know to take the average area of my working stds or check stds? Am I entering the average area in alter sample once I make a custom field for it? or can Empower do that? Also, what do you mean by adding the "%" in the custom fields above?

Thank you for the help!

[aceto_81]

We use the control sample type to do our standard verification.
You can average them in your report.

Ace

[ydna6969]

Hi
If you follow the custom field wizard in Empower then this is straight forward.
Click between standards and unknown & standards in alter table for entering other values

The '%' is a multiple number character wildcard for empower :

User-defined mathematical calculation summarizing samples

Label.Injection.Channel.Summary Function(Field)

So in this case Label =A or B
Injection, Channel is not important then add summary function. (IE A.%..then function (field))

I recommend trying the custom field wizard along with manual/excel calculations to verify custom fields
I have a number of custom field examples if you require further information

[nattasha]

Hi again

I've created all 5 custom fields, and they seem to be working, as I can see results in the Review window. I created them all as a peak type field. Any other field gives me an error. I'm wondering selecting the right type of field is important. (like sample, result, component, etc). I'm having a hard time getting the report method to give me my one value I need for % difference: % difference = ((Average Area of check std (n=2) x Wt std 1 X 100) / (Average Area of working std (n=6) x Wt std 2) - 100).

I've added my custom field: Percent_Difference to the all peaks table in the report method, but I get nothing. I believe I have all the required fields in the table, like sample name, area, peak type, label. How do I get Empower to give me 1 value? It's currently showing a result for % difference for each injection of my 6 Working standards in Review.

Any help would be greatly appreciated!

[nattasha]

Hi ydna6969

I ended up figuring out why I wasn't getting a result for % difference in my report method. The 5 custom fields you gave me - are they all supposed to be peak type fields? No result type correct? Also, I have created a 6th custom field, result type, data type: BOOL, to give me a Pass or Fail. This is my BOOL custom field: NLT(Average(Percent_Difference),10). It forced me to put the "average" in there. Why does it do this?

Can you or someone explain the values 0,1 and translations table? I have 0 as pass, and 1 as Fail, but no idea what the "0" or "1" mean... I got my report method to pass/fail, but it's giving me a fail incorrectly. It should say pass.

Thank you

[ydna6969]

Hi nattasha,

the 5 custom fields should be field type 'Peak' and type 'real'

I dont often use Boolean custom fields but best practice is to have a translation as you suggested to return one or two expressions.
I think you may have the BOOL custom field the opposite to what you require since it is reporting 'fail'

Again I dont use Boolean fields much ,, extra step which is not really required ,but can be helpful when reporting degs