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gradient for dissolution

http://www.chromforum.org/viewtopic.php?t=22412

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gradient for dissolution

Posted: Sun Apr 14, 2013 9:04 pm
by gerapas
could someone explain to me why one would do a 30 minute gradient to determine the dissolution of a tablet? The R.T. is about 20min and the peak is huge(no other peaks are present, very good baseline)

Re: gradient for dissolution

Posted: Mon Apr 15, 2013 8:02 am
by HPLCaddict
could someone explain to me why one would do a 30 minute gradient to determine the dissolution of a tablet? The R.T. is about 20min and the peak is huge(no other peaks are present, very good baseline)
Basically 2 possibilites:
- Some other peaks *could* be present (degradation or synthesis byproducts of the API) that need to be separated by that gradient
- The method is just too complicated, as already supposed by you :wink:

Re: gradient for dissolution

Posted: Mon Apr 15, 2013 8:20 am
by danko
If the method is identical with the one you use in assay context, it makes sense using it in dissolution testing as well.

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