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SDS and high salt concetration probes in HiTrap Protein G HP

http://www.chromforum.org/viewtopic.php?t=22062

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SDS and high salt concetration probes in HiTrap Protein G HP

Posted: Tue Mar 05, 2013 10:46 am
by Ana12
We would like to use HiTrap Protien G HP for our probes. They have a high salt concentration and might contain SDS. Unfortunately we didn't find any information about this from the manufacturer... Could these facts have negative effects on the column?

Thank you for your help!

Re: SDS and high salt concetration probes in HiTrap Protein

Posted: Wed Mar 06, 2013 8:30 am
by Gerhard Kratz
SDS should not be a problem, but high salt concentration can effect the binding capacity. Manufacturer recommends to use Desalting columns before the Protein G HP column. Maybe that will help. Good luck
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