SDS and high salt concetration probes in HiTrap Protein G HP

[Ana12]

We would like to use HiTrap Protien G HP for our probes. They have a high salt concentration and might contain SDS. Unfortunately we didn't find any information about this from the manufacturer... Could these facts have negative effects on the column?

Thank you for your help!

[Gerhard Kratz]

SDS should not be a problem, but high salt concentration can effect the binding capacity. Manufacturer recommends to use Desalting columns before the Protein G HP column. Maybe that will help. Good luck