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application for Polysorbate 20
Posted: Thu Feb 21, 2013 4:05 am
by amirtamboli
want to quantify poly20 with non-ELSD method
anybody have a solution for same
Re: application for Polysorbate 20
Posted: Thu Feb 21, 2013 2:50 pm
by max_planck
want to quantify poly20 with non-ELSD method
anybody have a solution for same
As part of a matrix (i.e. product)?
Re: application for Polysorbate 20
Posted: Thu Feb 21, 2013 3:48 pm
by Consumer Products Guy
10% or 10ppm? What matrix?
Re: application for Polysorbate 20
Posted: Fri Feb 22, 2013 4:23 am
by amirtamboli
5 ppm
Re: application for Polysorbate 20
Posted: Fri Feb 22, 2013 4:26 am
by amirtamboli
want to quantify poly20 with non-ELSD method
anybody have a solution for same
As part of a matrix (i.e. product)?
yes as a part of matrix with 5 ppm conc.
Re: application for Polysorbate 20
Posted: Fri Feb 22, 2013 9:31 am
by Gerhard Kratz
A nice application you can find under:
http://www.chem.agilent.com/Library/app ... 8311EN.pdf
I'm sure from other manufacturers you will find also some application notes on Tween 20.
Good luck
Re: application for Polysorbate 20
Posted: Fri Feb 22, 2013 12:29 pm
by amirtamboli
10% or 10ppm? What matrix?
5 ppm
Re: application for Polysorbate 20
Posted: Fri Feb 22, 2013 12:31 pm
by amirtamboli
thanks dear
bur its elsd method, i have tried and its not suitable for low concentration approx 5 ppm.
Re: application for Polysorbate 20
Posted: Sun Nov 12, 2017 10:59 am
by goran123
Hi,
I would like to analyze polysorbate 20 at a concentration of 0.05 mg/ml, or slightly lower, in a solution that contains protein, 10 mg/ml. If possible, I would like to use a simple method, including hydrolysis of PS20 by KOH followed by RPC analysis of the released lauric acid and quantification by using UV absorbance at 195-200 nm.
Is this possible?
Have you seen published methods that describes this? References?
I suppose that alternatives are including fluorescence detection after labelling of the released fatty acid? Do you have references to useful methods?
I am grateful for your input.
Best regards,
Goran Karlsson, PhD
Xbrane Biopharma
Sweden