application for Polysorbate 20

[amirtamboli]

want to quantify poly20 with non-ELSD method
anybody have a solution for same

[max_planck]

want to quantify poly20 with non-ELSD method
anybody have a solution for same

As part of a matrix (i.e. product)?

[Consumer Products Guy]

10% or 10ppm? What matrix?

[amirtamboli]

5 ppm

[amirtamboli]

want to quantify poly20 with non-ELSD method
anybody have a solution for same

As part of a matrix (i.e. product)?

yes as a part of matrix with 5 ppm conc.

[Gerhard Kratz]

A nice application you can find under: http://www.chem.agilent.com/Library/app ... 8311EN.pdf
I'm sure from other manufacturers you will find also some application notes on Tween 20.
Good luck

[amirtamboli]

10% or 10ppm? What matrix?

5 ppm

[amirtamboli]

A nice application you can find under: http://www.chem.agilent.com/Library/app ... 8311EN.pdf
I'm sure from other manufacturers you will find also some application notes on Tween 20.
Good luck

thanks dear
bur its elsd method, i have tried and its not suitable for low concentration approx 5 ppm.

[goran123]

Hi,
I would like to analyze polysorbate 20 at a concentration of 0.05 mg/ml, or slightly lower, in a solution that contains protein, 10 mg/ml. If possible, I would like to use a simple method, including hydrolysis of PS20 by KOH followed by RPC analysis of the released lauric acid and quantification by using UV absorbance at 195-200 nm.
Is this possible?
Have you seen published methods that describes this? References?

I suppose that alternatives are including fluorescence detection after labelling of the released fatty acid? Do you have references to useful methods?

I am grateful for your input.

Best regards,

Goran Karlsson, PhD
Xbrane Biopharma
Sweden