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gradient for faropenem

http://www.chromforum.org/viewtopic.php?t=2194

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gradient for faropenem

Posted: Fri Jul 08, 2005 5:39 am
by dhirren
dear friends ,
i am developing gradient elution for faropenem sodium using MEOH and ammonium acetate buffer but the peak has too much fronting. do i need to change the teepness of the gradient?
yours
dhiren[/list]

Posted: Fri Jul 08, 2005 3:01 pm
by MG
If your sample solvent contains more organic solvent than your initial mobile phase, try either reducing the percentage of organic solvent in your samples, or injecting a smaller volume.
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