gradient for faropenem

[dhirren]

dear friends ,
i am developing gradient elution for faropenem sodium using MEOH and ammonium acetate buffer but the peak has too much fronting. do i need to change the teepness of the gradient?
yours
dhiren[/list]

[MG]

If your sample solvent contains more organic solvent than your initial mobile phase, try either reducing the percentage of organic solvent in your samples, or injecting a smaller volume.