front tailing

[push]

Which factors can cause a front tailing in HPLC?

[Vlad Orlovsky]

for some basic compounds it can be overloading of residual silanols at certain pH

[push]

Thnks Vlad,
But the analyte does not contain nor basic neither acid groups.

[Consumer Products Guy]

Inject less microliters, see if it gets better.

[Vlad Orlovsky]

reasons:
overloading
poorly packed column (with fronting to help peaks with tailing)
diluent effect
solubility effect
tautomerism of compound (not defined "batman peak" for example)

[tom jupille]

There is a chalk-talk that covers that:
viewtopic.php?f=31&t=18426
Fronting is discussed near the end (about 8 minutes in), but it's best to watch it all to put fronting into perspective.