-
- Posts: 1
- Joined: Tue Jan 29, 2013 10:59 pm
Advertisement
Filtering Sulfuric Acid Mobile Phase
Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.
2 posts
Page 1 of 1
I am relatively new to HPLC. I will be attempting to separate oligosaccharides on a BioRad Aminex HPX-87H column with a Micro-Guard Cation H+ cartridge. I plan to use the NREL procedure for this separation found here: http://www.nrel.gov/biomass/pdfs/42623.pdf . It uses a 5 mM H2SO4 mobile phase. If this solution is made with water from a millipore synergy system (18 megaohm resistance), do I need to prefilter the solution? If so, what method would you reccomend?
-
- Posts: 3594
- Joined: Mon Aug 30, 2004 11:43 pm
We make up dilute sulfuric acid here using Millipore water and a few ml of dilute purchased sulfuric acid titrant; we do not filter this before use.
We filter when we dissolve a solid in the HPLC solvent.
We filter when we dissolve a solid in the HPLC solvent.
2 posts
Page 1 of 1
Who is online
In total there are 36 users online :: 0 registered, 0 hidden and 36 guests (based on users active over the past 5 minutes)
Most users ever online was 4374 on Fri Oct 03, 2025 12:41 am
Users browsing this forum: No registered users and 36 guests
Most users ever online was 4374 on Fri Oct 03, 2025 12:41 am
Users browsing this forum: No registered users and 36 guests
Latest Blog Posts from Separation Science
Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.
Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.
- Follow us on Twitter: @Sep_Science
- Follow us on Linkedin: Separation Science
