GPC Polystyrene Standard Not Linear
Posted: Fri Dec 28, 2012 9:59 pm
Hi all,
Happy Holidays!
I have a questions on GPC analysis with Agilent 1100 system. I run the system with easiVial polystyrene standards, THF as solvent, the column is resipore with a resipore guard column, detector is DAD. Injection volume is 90ul, flow rate is 1ml/min. The peaks were good. However, when I correlate the MW with retention time, it is NOT linear! Instead, it is exponential. Any ideas of what was wrong?
Thank you very much.
Jen
Happy Holidays!
I have a questions on GPC analysis with Agilent 1100 system. I run the system with easiVial polystyrene standards, THF as solvent, the column is resipore with a resipore guard column, detector is DAD. Injection volume is 90ul, flow rate is 1ml/min. The peaks were good. However, when I correlate the MW with retention time, it is NOT linear! Instead, it is exponential. Any ideas of what was wrong?
Thank you very much.
Jen