Analytical recovery vit E determination HPLC-Fluoremetric

[montana]

Hi,

The determination of vit E in Plasma needs extraction , so i prepare a standard solution of vit E and calibration curve using different concentration of vit E in ethanol ( 5-40 mg/l) , after that i prepar the same standard solution for the calibration curve but i replace the ethanol by plasma for the prerpartion, after extraction and analysis by HPLC-fluo .

The first calibration curve (vit E in ethanol) and the second calibration curve were ploted.

Can you plz help me , how recovery can be determinate ?

BR.

[schlepro]

Dear BR,

I would try using solvents for extraction that vitamin E is soluble in.

Sincerely,
Keith

[upamaniah]

use Shodex ODP2HP column for better recovery from plasma sample.
any way,
compare the area between ethanol and plasma for the same concentration, and consider area in ethanol is 100%.
(area of vit E in Plasma X 100)/area of vit E in ethanol could give roughly the % recovery at each concentration.

good luck

[montana]

Thank you