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Startup procedure after changing rotor seal

http://www.chromforum.org/viewtopic.php?t=21269

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Startup procedure after changing rotor seal

Posted: Mon Nov 12, 2012 4:27 am
by Chromato86
Hi,

I had to chang the rotor seal in the injection Valve and now I need to startup the whole HPLC system to get the air out of it. Does anyone know how to proceed or can give me a link? I have been looking for a long time but found no description how to proceed.

The HPLC system is from SHIMADZU with the following components:

- LC-20AD pump
- RID-10A detector
- DGU-20A3 solvent degasser
- CTO-20A oven
- 6 position injection valve (Rheodyne) and an Aminex column

I would be very glad if anyone could provide help!

Re: Startup procedure after changing rotor seal

Posted: Fri Jan 11, 2013 9:09 pm
by uzman
Simply , disconnect the column inlet , change the rheodyne valve to inject position , start the pump and adjust the flow rate to 3 ml/min , wait 10 seconds and connect the column again .

Re: Startup procedure after changing rotor seal

Posted: Sat Jan 12, 2013 6:51 pm
by uzman
Sorry , I forgot to mention :
Adjust the flow rate to appropriate value before connecting the column.

3ml/min flow is too high for analytical column.

Re: Startup procedure after changing rotor seal

Posted: Sun Jan 13, 2013 4:42 am
by Chromato86
Thank you very much!
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