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FORMIC ACID VERSUS TFA

http://www.chromforum.org/viewtopic.php?t=21205

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FORMIC ACID VERSUS TFA

Posted: Thu Nov 01, 2012 5:19 pm
by shemesh199
Hello all,

Generally speaking , can 0.1% formic acid be used as the aqueous mobile phase instead of 0.1% TFA in regards to peptide analysis by ESI-MS-MS?

Thanks

Re: FORMIC ACID VERSUS TFA

Posted: Thu Nov 01, 2012 5:24 pm
by JMB
Absolutely !!!!!!!!!!!!

Will give stronger [M + nH]n+ signals than TFA, which forms very strong ion pairs (neutral).

JMB
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