Can Drylab model Ion Chromatography

[Paulhurleyuk]

I'm doing some Ion Chromatography development (Dionex ICS-3000 RFIC, KOH mobile phase, AS-11 4-250mm column). As I would do for Reversed Phase, I ran some scouting gradients (of KOH molarity instead of % organic) and threw the data in Drylab. Drylab has produced some predictions which seem to make sense, and seem to tally up against some furthur experiments, but I'm wondering if that's just a fluke.

Is Drylab capable of modelling Ion Chromatography ? Is what I've done scientifically acceptable ?

Thanks

Paul.

[aceto_81]

You can even define your own models, so why not?

It is however possible that KOH molarity shows another behaviour as %organic, so it might be usefull to use another mode as %organic.


HTH

Ace

[tom jupille]

The underlying model (linear solvent-strength-gradients) in DryLab is not ideal for ion exchange, but in practice it works well enough. That was demonstrated back in 1989 (Sasegawa, Sakamoto, Hirose, Yoshida, Kobayashi, & Sato; J. Chromatog., 485, 533-540 (1989)). The average error using the "correct" model was 0.6%; the average error using DryLab was 0.8%.

[John Lim]

Have you considered Virtual Column from Thermo Fisher Dionex since you have an ICS-3000 and Chromeleon? This Chromeleon add-in is an IC simulator based on empirical data for anions, cations and carbohydrates with analyte specific retention algorithms for Dionex specific columns. The integrated Application Wizard will export your modeling results directly into your Chromeleon PGM and QNT files. I can verify that your AS-11 4 mm column is listed in the VC database.

http://www.dionex.com/en-us/products/ch ... 81478.html

Hope this helps!

John