Dear All,

I am doing assay for Azithromycin as per EP 7.0. The problem is with repeatability and base line stabilization.
Area is varying from injection to injection.

The condition i applied are mention below for the reference
Column: Asahipak ODP-50; 250 x 4.6mm; 5µ
Temp: 40°C
Flow: 1.0mL/min
Mobile Phase: 60 Vol ACN : 40 Vol of 6.7g/L of dipotassium hydrogen phosphate (pH adjusted to 11.0 with 560g/L solution of KOH)
Wavelength : 210nm
Sample preparation in: 60 Vol of ACN : 40 Vol of 6.7g/L solutin of dipotassium hydrogen phosphate (pH adjusted to 8.0 with phosphoric acid)

Looking forward for your help.

Regards
M