peak height of chromatograph

[bobey]

hi. I'm using GC-FID GC-14B SHIMADZU.

I used Program : GCSOLUTION SHIDMADZU for data integration. My problem is the magnifying factor or 'zooming factor' of chromatogram which is I found really difficult to understand. Since I used the '+' and '-' button of the program, (refer to picture 1), how is the real magnifying factor of my chromatograph since I found the information from GC reference (pg.95) saying that "When the [+] button is clicked on, the chromatogram height will be enlarged two times larger(the intensity axis scale will become half in length). When the [-] button is clicked on, the chromatogram height will be reduced to half (the intensity axis scale will become two times
longer)."

Q1: DO I NEED TO CONSIDER THIS INFORMATION IN ORDER TO DETERMINE THE 'ZOOMING' FACTOR OF MY CHROMATOGRAPH?

if i am going to paste picture 1 in my paper for publication, is that right to write "the chromatograph is ATTENUATED BY 2000 times".[magnifying power x 2 (by using the zooming factor "x1000" from the chromatograph and the times by 2 since the height will be enlarged two times]

is this correct???

Q2: By looking at the result peak table (refer picture 3), refer row 2, the retention time is 10.329 and the height is 853. what is the unit of this height???? is that micro-Volt (uV). thus, is it the SAME to say the height of the peak at retention time 10.329 min is (8.53 x 10^-4) Volt ???? since 1micro = 1 x 10^-6


your help is highly appreciated.

thanx.

regards,
bobey

picture1 http://s825.photobucket.com/albums/zz17 ... %3Dfid.jpg

picture 2 http://s825.photobucket.com/albums/zz17 ... 3Dfid2.jpg

picture 3 http://s825.photobucket.com/albums/zz17 ... 3Dfid3.jpg

[Peter Apps]

hi. I'm using GC-FID GC-14B SHIMADZU.

I used Program : GCSOLUTION SHIDMADZU for data integration. My problem is the magnifying factor or 'zooming factor' of chromatogram which is I found really difficult to understand. Since I used the '+' and '-' button of the program, (refer to picture 1), how is the real magnifying factor of my chromatograph since I found the information from GC reference (pg.95) saying that "When the [+] button is clicked on, the chromatogram height will be enlarged two times larger(the intensity axis scale will become half in length). When the [-] button is clicked on, the chromatogram height will be reduced to half (the intensity axis scale will become two times
longer)."

Q1: DO I NEED TO CONSIDER THIS INFORMATION IN ORDER TO DETERMINE THE 'ZOOMING' FACTOR OF MY CHROMATOGRAPH? As long as the axes and their units appear on the figure then the "zooming factor" is irrelevant

if i am going to paste picture 1 in my paper for publication, is that right to write "the chromatograph is ATTENUATED BY 2000 times".[magnifying power x 2 (by using the zooming factor "x1000" from the chromatograph and the times by 2 since the height will be enlarged two times]include the axes and their units,and do not mention the attenuation

is this correct???

Q2: By looking at the result peak table (refer picture 3), refer row 2, the retention time is 10.329 and the height is 853. what is the unit of this height???? is that micro-Volt (uV). thus, is it the SAME to say the height of the peak at retention time 10.329 min is (8.53 x 10^-4) Volt ???? since 1micro = 1 x 10^-6Formally it is correct, but 853 uV is also correct, and if your wish to use 8.53 x 10^-4 is motivated by concern about strict adherence to the SI units, then you need to convert the retention time to seconds


your help is highly appreciated. you need to study the style requirements of the journal that you intend to publish in

thanx.

regards,
bobey

picture1 http://s825.photobucket.com/albums/zz17 ... %3Dfid.jpg

picture 2 http://s825.photobucket.com/albums/zz17 ... 3Dfid2.jpg

picture 3 http://s825.photobucket.com/albums/zz17 ... 3Dfid3.jpg

[bobey]

thanx for the explanation but i still want to know what is the purpose of (x1,000) on the y-scale of the chromatogram in picture 1. it seems to change (increasing or decreasing to the power of 10) when i click "+" or "-" buttons... is that zooming factor???? how can i calculate it then???

[GCMSNoob]

All you are doing is zooming the view of the chromatogram. The scale of the y-axis decreases as you zoom in. All the (x1000) is is the scale of the axis. No calculations necessary.