Chromatography Forum

I am using an Agilent 1200 Fraction collector, and it consistently skips the second fraction after a run is started. It doesn't matter where in the plate the run is started, the second fraction is skipped. I have looked all through the method (ChemStation), and even started from a default method. There is no setting to skip a fraction anywhere I can find. Any suggestions?

Anyone have any ideas?

Has anyone ever seen this?

Hi mmanders,
I have just looked into this and found a service note that might cause your problem. It is related to the G1315C/D or G1365C/D firmware causing incorrect fraction trigger under certain circumstances.
Newer FW versions should overcome this problem (FW > B06.13)
As a temp solution you could select threshold or slope only as trigger modes, or specify a threshold that is significant above baseline to prevent a trigger.

Cheers, René Blom

Thanks for your response. Do you know if this FW error would also affect fractions collected by time, not peak detection?

Hi, the svc note only mentions peak based fractionation, so I expect that if you collect time-based the problem doesn't occur

It is actually time-based collection that we are doing when we have the problem. Our techs have reinstalled and updated to the latest firmware without any luck.

still sounds like the firmware/driver is using a different vial position vs. what the software thinks. Does it skip vial 2 then it moves on (1,3,4,5,6,7...), or does it increment every 2 vials ex: 1, 3, 5, 7...

try to find where firmware/driver gets vial position information.