Agilent 1200 Fraction Collector skipping fraction

[mmanders]

I am using an Agilent 1200 Fraction collector, and it consistently skips the second fraction after a run is started. It doesn't matter where in the plate the run is started, the second fraction is skipped. I have looked all through the method (ChemStation), and even started from a default method. There is no setting to skip a fraction anywhere I can find. Any suggestions?

[mmanders]

Anyone have any ideas?

[mmanders]

Has anyone ever seen this?

[RBlom]

Hi mmanders,
I have just looked into this and found a service note that might cause your problem. It is related to the G1315C/D or G1365C/D firmware causing incorrect fraction trigger under certain circumstances.
Newer FW versions should overcome this problem (FW > B06.13)
As a temp solution you could select threshold or slope only as trigger modes, or specify a threshold that is significant above baseline to prevent a trigger.

Cheers, René Blom

[mmanders]

Thanks for your response. Do you know if this FW error would also affect fractions collected by time, not peak detection?

[RBlom]

Hi, the svc note only mentions peak based fractionation, so I expect that if you collect time-based the problem doesn't occur

[mmanders]

It is actually time-based collection that we are doing when we have the problem. Our techs have reinstalled and updated to the latest firmware without any luck.

[5.56x45mm]

still sounds like the firmware/driver is using a different vial position vs. what the software thinks. Does it skip vial 2 then it moves on (1,3,4,5,6,7...), or does it increment every 2 vials ex: 1, 3, 5, 7...

try to find where firmware/driver gets vial position information.