Impurity caculation using RRF

[pjalindar]

is RRF multiply or divide to calculate impurity

[imran_ali_qu]

Yes it is very essential to keep the RRF for impurity calculation. and it will divide from calculation..

[HPLCaddict]

It depends on how you calclulated the RRF. May be both ways...

[tom jupille]

As HPLCaddict pointed out, it can be defined either way.

If it is defined as the RRF of the impurity (the most common situation), it is used as a divisor. In that case, the RRF is ratio of responses: Impurity/Parent. Let's suppose your impurity has an RRF of 0.5. That means that for equal amounts, the impurity peak will be half as big as the parent peak. You divide by the RRF to correct for that.

If it is defined as the RRF of the parent, then you would multiply.