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HPLC column pressure problem

http://www.chromforum.org/viewtopic.php?t=20172

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HPLC column pressure problem

Posted: Wed Jun 27, 2012 4:50 pm
by Xinji Li
Hi,

My HPLC column (CA-5ODS) for determination of catacholamines in blood sample is becoming worse and worse. the more use , the more column pressure increase. How to resolve this problem? Thanks for your suggestion in advance.

Re: HPLC column pressure problem

Posted: Thu Jun 28, 2012 1:00 am
by tom jupille
Eicom apparently sells their systems as "turn-key" solutions. Their web site provides no information about the column chemistry. Anything I can recommend would be general (e.g., backflush the column) and might damage the column further. The best suggestion I can make is to contact them for support.
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