qualification of Choline bitartrate by HPLc

[phucto]

Dear Sirs
I was assigned to develop the HPLC method for assay of Choline bitartrate in soft capsule (consists of Sodium chondroitin sulfate, Vitamin B2 and B6) with detector UV or RID (My lab is available two these detectors)

I looked throughout the papers but there is method with conductivity detector.
Anyone help me with this method?

Thank you very much!

[tom jupille]

Direct (non-suppressed) conductivity detectors are cheap. It might well cost you less to get the appropriate detector than to spend the time to work around the lack of it.

That said, if it's an isocratic method, you should be able to make RI work, but you will have limited sensitivity.