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equilibration time Vs stationary phase ligand ?
Posted: Sun Jun 17, 2012 7:17 pm
by rick1112
hi
does the equilibration time with respect to type of stationary phase ligand ??
eg..does C18 get equlibrated at the same time as C8 or C4 ??
Re: equilibration time Vs stationary phase ligand ?
Posted: Mon Jun 18, 2012 2:43 am
by tom jupille
To a first approximation, yes. Where you would be more likely to see a difference is between monomeric and polymeric phases.
Re: equilibration time Vs stationary phase ligand ?
Posted: Mon Jun 18, 2012 8:23 pm
by rick1112
thanks tom,
what is the reason for such difference in time, ??
on a different note, are there many polymeric columns out there ? , mainly i have come across monomeric columns
i kind of thought that polymeric columns suffer from reproducibility issues
Re: equilibration time Vs stationary phase ligand ?
Posted: Mon Jun 18, 2012 9:21 pm
by tom jupille
what is the reason for such difference in time, ??
Slow diffusion in that thick greasy, crosslinked bonded phase layer.
on a different note, are there many polymeric columns out there ? , mainly i have come across monomeric columns
i kind of thought that polymeric columns suffer from reproducibility issues
They do have problems, and that's why there are very few of them. The ones that do exist were largely developed decades ago as a workaround for silanol-related tailing.