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old DEAE sepharose regeneration

http://www.chromforum.org/viewtopic.php?t=1970

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old DEAE sepharose regeneration

Posted: Wed May 18, 2005 12:27 am
by jet66
I am cleaning my DEAE cloumn, it is a very old cloumn, the media has been using at least 1000 cycles. the media is now look greenish colour, it has been using in bovin albumin manufacturing. Any body know how to regenerate this media?

thanks
jet

Posted: Wed May 18, 2005 2:19 am
by tom jupille
Check with the manufacturer (but if it's really turning green, it may be time to replace :wink: ).
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