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Column for EDTA

http://www.chromforum.org/viewtopic.php?t=18824

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Column for EDTA

Posted: Wed Jan 18, 2012 4:51 pm
by frasmx
Hello, it wanted they were recommending to me columns for the analysis of eDTA by HPLC?, and that conditions are adapted to realize the analysis, regards and thanks.

Re: Column for EDTA

Posted: Thu Jan 19, 2012 12:23 am
by tom jupille
EDTA has been done by anion exchange, by reversed-phase, and by mixed-mode columns. Do a Google search; you'll find lots of methods.

Re: Column for EDTA

Posted: Thu Jan 19, 2012 3:33 pm
by Consumer Products Guy
We've assyed readily for EDTA in consumer products using Cu+2 in the mobile phase and the dilution solvent, using UV detector and RP-8 columns.

Re: Column for EDTA

Posted: Thu Jan 19, 2012 10:53 pm
by Vlad Orlovsky
Here are methods which were validated in 3 pharma companies in US. Cu sulfate is used for visualization. Peaks are extremely sharp due to focusing effect:
http://www.sielc.com/Compound-EDTA-Ethy ... -Acid.html

contact me if you have questions.
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