Column for EDTA

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

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Column for EDTA

: frasmx

: Posts: 19; Joined: Tue Jul 13, 2010 5:19 pm

by frasmx » Wed Jan 18, 2012 4:51 pm

Hello, it wanted they were recommending to me columns for the analysis of eDTA by HPLC?, and that conditions are adapted to realize the analysis, regards and thanks.

Re: Column for EDTA

: tom jupille; Site Admin

: Posts: 4978; Joined: Wed Aug 11, 2004 4:55 pm

by tom jupille » Thu Jan 19, 2012 12:23 am

EDTA has been done by anion exchange, by reversed-phase, and by mixed-mode columns. Do a Google search; you'll find lots of methods.

-- Tom Jupille
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374

Re: Column for EDTA

: Consumer Products Guy

: Posts: 3594; Joined: Mon Aug 30, 2004 11:43 pm

by Consumer Products Guy » Thu Jan 19, 2012 3:33 pm

We've assyed readily for EDTA in consumer products using Cu+2 in the mobile phase and the dilution solvent, using UV detector and RP-8 columns.

Re: Column for EDTA

: Vlad Orlovsky

: Posts: 775; Joined: Mon Dec 01, 2008 9:59 pm

by Vlad Orlovsky » Thu Jan 19, 2012 10:53 pm

Here are methods which were validated in 3 pharma companies in US. Cu sulfate is used for visualization. Peaks are extremely sharp due to focusing effect:
http://www.sielc.com/Compound-EDTA-Ethy ... -Acid.html

contact me if you have questions.

Vlad Orlovsky
HELIX Chromatography
My opinions might be bias, but I have about 1000 examples to support them. Check our website for new science and applications
www.helixchrom.com

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