HPLC gradient pump checking?

[ph_yasir]

How could we determine whether or not the gradient pump is generating a gradient? Is there more than one method?

[tom jupille]

Use the conditions for the dwell volume measurement:
http://www.lcresources.com/resources/TSWiz/hs410.htm

[Sallybeetle]

I have instituted John Dolan's gradient performance checks in two different laboratories, and on many different HPLC systems. These checks are included in the article below.

Dolan, J., et al, "Gradient Performance Checks," LCGC North America, Volume 22, Number 10, October 2004.
http://www.chromatographyonline.com/lcg ... rticle.pdf

[lmh]

... and on a related issue I am KICKING myself for specifying an LC-MS system without any UV detection, with a strong motivation behind my kicking being that I have no easy way to check its pump.

[rhaefe]

...and on another related issue I know quite a few people who by default use a conductivity detector after the UV (or fluorescence) detector to monitor the gradient (works best if there is a buffer of some sort involved).

[Sallybeetle]

... and on a related issue I am KICKING myself for specifying an LC-MS system without any UV detection, with a strong motivation behind my kicking being that I have no easy way to check its pump.

Perhaps you could purchase an inexpensive, used detector for performance checks; e.g., gradient check, precision and linearity checks, etc.