Some queries of HLPC. Please help me!

[ultima_86]

Hello everyone, I am so grateful to you for answering these questions as well as advice from you.
1. I analyze Metformin with HPLC and recieved a long - tailng peak, therefore I added a little Trimethylamine to my buffer to shorten my peak's tailing. It appears to work, my peak becames better. But I wonder if there are some problems happen beside this good side?

2. Can elements of mobile phase (pH, composition ...) increase or decrese peak's area?

[Gerhard Kratz]

To add TMA will give you better peak shape. Beside that you can try a more base buffer or columns with lower silanol activity.
As long as you separate standard and sample under the same conditions you are on the save side. Good luck.

[ultima_86]

Thank to Gerhard Kratz for your answer. This helps me a lot!

[amirnimo]

What is the method you are using for analysis of Metformin?

I need help for a similar analysis.

I tried to use ion pair like Heptane sulfonic acid and TEA with no effect.

Can I use TFA 0.1% ?

What column type are you using?

Please help.

The original post viewtopic.php?f=1&t=17994

[Vlad Orlovsky]

Here is a method with perfect peak shape and retention control. Metformin is retained by combination of weak reversed-phase and strong cation-exchange mechanisms:
http://www.sielc.com/Compound-Metformin.html

Contact me if you have questions.