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- Posts: 37
- Joined: Tue Feb 20, 2007 12:36 pm
I am writing a validation plan for a GC method and was wondering what robustness parameters you would usually validate for such a method?
The chromatographic conditions are as follows:
Column: DBWAX column of 30 m x 0.32 mm i.d., 0.5 µm film thickness
Carrier gas: Nitrogen
Flow rate: 1 ml/min, with 1:35 split ratio
Purge: 4.0 ml/min
Detection: FID at 250 ºC
Gas used for detection: Hydrogen at a flow rate of 44 ml/min
Air at a flow rate of 275 ml/min
Nitrogen at a flow rate of 20/min
Injection method: Split flow, maintained at 200 °C, with 1 µl injection volume
and unpacked insert
Temperature: Isotherm at 60 °C
Run time: 20 minutes
So far I would change the injector, detector and column temperature by +/- 10%.
But some of the parameters above do not make sense to me, such as the 4ml/min purge. Does anyone know if this is a headspace method and can I run this on an Agilent system too?
Thanks for any help in advance!
Max