Deuterated Analogue as internal standard

[joel.ch]

Hello, great to find such a community...

I'm wondering on the effect of different deuterated analogues (d3, d6, ...) on the tR's. Couldn't find anything consistent on the web nor the forum.

The goal is to have the same (or closest) tR during a LC-MS run (to get a simultaneous ionisation), and I cannot decide between the different available species.

Thanks to anybody who might help me solving this...

Joël

Datas: LC-MS 1100-MSD, C18 column and MeOH/H2O mobile phase (formic acid/sodium formate)

[MG]

In LC-MS I have seen anywhere from no difference, to a very slight difference in retention times, for deuterated versus non-deuterated. It is not something I would worry about. [/i]

[joel.ch]

First of all, excellent that this place is so active...

Thanks for your answer, I have the choice to buy the d3 or d6 analogue of my product which has a Mw of about 1200 g/mol... Considering your answer, I'll go on for the d6, avoiding isotopic distribution influence that could occur with the d3.

Joël

[Ron]

There is generally very little differences in retention time with the deuterated compounds, so look for spectral interferences as you noted above, then consider cost and availability.