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- Posts: 45
- Joined: Tue Aug 09, 2011 9:16 am
I have a little bit of a problem with the Chromsystems B1/B6 HPLC kit and I thought you might help me.
I want to use the kit but it says that I need to switch the mobile phases at 2.5 min. Does anyone know if the switch is gradual or I just switch from 100% mobile phase A to 100% mobile phase B.
As for detection it is said that I need to switch at 4 min from one EX/EM setting to another. Can I just use an interval instead of switching between the two or it is mandatory to use the exact settings mentioned in the prospect.
Thank you in advance