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- Posts: 2
- Joined: Wed Jul 27, 2011 3:02 pm
hi guys,
When ever i am using gradient program i always used to find lot of peaks.
not only at the sarting and ending of graient program.
EXAMPLE: I have a mixture of highly polar, medium polar and non polar compounds to separate
A:Buffer (KH2PO4)
B:ACN or methanol
Time %A %B
0 100 0
10 100 0
30 20 80
50 20 80
60 100 0
65 STOP
problem is these small gradient peaks interfering my non polar impurities at 0.1% Level
and some times not reproducible.
Do u think anyof these following reasons
1.instrument make
2.Gradient ramp
3.Solvent/Buffer grades
4.Premixing of organic to buffer.
PLEASE ADVISE
Regards
Krish
When ever i am using gradient program i always used to find lot of peaks.
not only at the sarting and ending of graient program.
EXAMPLE: I have a mixture of highly polar, medium polar and non polar compounds to separate
A:Buffer (KH2PO4)
B:ACN or methanol
Time %A %B
0 100 0
10 100 0
30 20 80
50 20 80
60 100 0
65 STOP
problem is these small gradient peaks interfering my non polar impurities at 0.1% Level
and some times not reproducible.
Do u think anyof these following reasons
1.instrument make
2.Gradient ramp
3.Solvent/Buffer grades
4.Premixing of organic to buffer.
PLEASE ADVISE
Regards
Krish
