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ITraq -Tryptic pep
Discussions about sample preparation: extraction, cleanup, derivatization, etc.
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I am preparing Tcell lysates for Itraq labeling. I need to remove detergents prior to trypsin trtmnt . And desalt prior to Hplc after Itraq labeling. I do not have extra sample to accommodate loss. Which is the simple//?best column for removing these from my samples (my total protein conc range between 30-120ugms).Pls suggest..
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