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extract and determine a Pharmaceuticals in oi
Posted: Sat Jul 02, 2011 10:20 pm
by mmajdop
hii,
i am traying to extract and determine a Pharmaceuticals in oil, can you help me please???????
i want to determine:
Ampicillin,Carbamazepine,ciprofloxacin,Sulfadimethoxine,Tetracycline,17b-estradiol.
thank you
Re: extract and determine a Pharmaceuticals in oi
Posted: Sun Jul 03, 2011 12:49 pm
by Axar
Hi!!!
You need to know properties of these medicines.
For example: Ampicillin has -NH2 and -COOH groups, badly soluble in water, stable in acidic solutions (data from wiki). In this case I would use aqueous solution with low pH to extract ampicillin from oil.
Also it would be usefull to add emulsifer to your oil then extracting
Re: extract and determine a Pharmaceuticals in oi
Posted: Sun Jul 03, 2011 9:18 pm
by mmajdop
thank you
can you suggest what kind of seperation column can i use?
Re: extract and determine a Pharmaceuticals in oi
Posted: Mon Jul 04, 2011 6:07 am
by Axar
I guess C18 is an option, but I do not familiar with HPLC.
Also you can change retention time of analite with mobile phase.
You can change pH, % of organic in phase, and add some buffer or/and ion-pair solts.
Re: extract and determine a Pharmaceuticals in oi
Posted: Mon Jul 04, 2011 7:07 am
by mmajdop
hii
thank you, i will try