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LC/MS help need
Posted: Tue May 31, 2011 12:31 pm
by kiransunku
Hello all,
I am going to do LC/Ms analysis of labled arginine which is incorporated in green algae (chlamydomonas). I feed labled argininie to algae and I want to know percentage of incorporation.
I want preparation protocal or related journals.
any help is appreciated.
Thanks
Kiran
Re: LC/MS help need
Posted: Tue May 31, 2011 2:57 pm
by lmh
There are loads of possible articles. Even amateurs like me have dabbled:
http://www.nxtbook.com/nxtbooks/advanstar/lcgc0406/
(if the link doesn't work for any reason, let me know; the article is the one on use of isotopes in LC-MS)
Re: LC/MS help need
Posted: Tue May 31, 2011 4:47 pm
by gxch43
http://www.biotechniques.com/multimedia ... _5240a.pdf
you can search for some vegetable sample preparation procedure as the reference of your algae sample.hope this help,
Re: LC/MS help need
Posted: Tue May 31, 2011 5:18 pm
by kiransunku
Thanks lmh,
the link you gave is discussed about the intepretation of peaks and theory. I need a method to prepare sample for LC/MS analysis.
Kiran
Re: LC/MS help need
Posted: Tue May 31, 2011 5:19 pm
by kiransunku
thanks gxch43
indeed the article is about arginine related thing
kiran
Re: LC/MS help need
Posted: Tue May 31, 2011 10:05 pm
by lmh
if you just want a method to extract and analyse arginine, you can buy a kit and instructions from almost any manufacturer. There are loads of ways to analyse amino acids, and Chlamy is a model species with a huge literature. Good luck!
Re: LC/MS help need
Posted: Wed Jun 01, 2011 7:50 am
by lmh
kiran, I owe you an apology for my last post which was ungenerous. I assumed from your first post that you were looking at incorporation of label into amino acids, which is indeed a subject of the reference I sent you. I was wrong, sorry!
Re: LC/MS help need
Posted: Thu Jun 02, 2011 6:09 am
by DSP007
There is a nuance.
Your task is to determine the arginine in the culture medium, in a mass of cells or directly into the organelle (chloroplast).?
Re: LC/MS help need
Posted: Thu Jun 02, 2011 12:10 pm
by kiransunku
My task is to find percentage of incorporation into membrane proteins (photosystem II). I used 98 % labled arginine to grow the culture algae.
Kiran