LC/MS help need

[kiransunku]

Hello all,

I am going to do LC/Ms analysis of labled arginine which is incorporated in green algae (chlamydomonas). I feed labled argininie to algae and I want to know percentage of incorporation.

I want preparation protocal or related journals.
any help is appreciated.

Thanks
Kiran

[lmh]

There are loads of possible articles. Even amateurs like me have dabbled: http://www.nxtbook.com/nxtbooks/advanstar/lcgc0406/
(if the link doesn't work for any reason, let me know; the article is the one on use of isotopes in LC-MS)

[gxch43]

http://www.biotechniques.com/multimedia ... _5240a.pdf

you can search for some vegetable sample preparation procedure as the reference of your algae sample.hope this help,

[kiransunku]

Thanks lmh,

the link you gave is discussed about the intepretation of peaks and theory. I need a method to prepare sample for LC/MS analysis.

Kiran

[kiransunku]

thanks gxch43

indeed the article is about arginine related thing

kiran

[lmh]

if you just want a method to extract and analyse arginine, you can buy a kit and instructions from almost any manufacturer. There are loads of ways to analyse amino acids, and Chlamy is a model species with a huge literature. Good luck!

[lmh]

kiran, I owe you an apology for my last post which was ungenerous. I assumed from your first post that you were looking at incorporation of label into amino acids, which is indeed a subject of the reference I sent you. I was wrong, sorry!

[DSP007]

There is a nuance.
Your task is to determine the arginine in the culture medium, in a mass of cells or directly into the organelle (chloroplast).?

[kiransunku]

My task is to find percentage of incorporation into membrane proteins (photosystem II). I used 98 % labled arginine to grow the culture algae.

Kiran