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- Posts: 16
- Joined: Thu Apr 08, 2010 12:14 am
Hi, I am trying to separate D/L enantiomers of alpha-methylbenzylamine. This compound is sometimes use as a standard for chiral separation, and there are methods out there. FYI, I do not have access to GC.
Some methods I found are:
Chiralpak OD-H (4.6x250) 1 mL/min, 9:1 Hex:IPA, 12 min. (210 nm)
Crownpak (Daicel) 0.7 mL/min, pure water (pH2), 16 and 19 min. (254 nm)
After acetylation,
Chiralcel AD-H (4.6 x 250), 0.5mL/min, 9:1 Hex:IPA.
The columns I have: Chiracel OD, OJ and Chiralpak AD.
So far I only have luck after acetylation, with Chiralpak AD using the same method reported.
But be nice if no acetylation needed. So I have been playing around with my OD and AD columns but have no luck with separation. I also have no idea how the first method was done, because I was never able to get a nice flat baseline at 210 nm.
Any advise?
Some methods I found are:
Chiralpak OD-H (4.6x250) 1 mL/min, 9:1 Hex:IPA, 12 min. (210 nm)
Crownpak (Daicel) 0.7 mL/min, pure water (pH2), 16 and 19 min. (254 nm)
After acetylation,
Chiralcel AD-H (4.6 x 250), 0.5mL/min, 9:1 Hex:IPA.
The columns I have: Chiracel OD, OJ and Chiralpak AD.
So far I only have luck after acetylation, with Chiralpak AD using the same method reported.
But be nice if no acetylation needed. So I have been playing around with my OD and AD columns but have no luck with separation. I also have no idea how the first method was done, because I was never able to get a nice flat baseline at 210 nm.
Any advise?
