Advertisement

HP3396 integrator trouble

Discussions about chromatography data systems, LIMS, controllers, computer issues and related topics.

4 posts Page 1 of 1
I have been working with an HP3396 Series II Integrator. It was working fine but recently started giving me trouble. I am seeing excessive noise or drift on the chromatogram when I should be seeing a baseline. I ran the tests as stated in the manual and it has passed them all. I know the power cable is fine because I swapped it for another and it works just as well. I also ran the T test and though the retention times of the peaks were the same, the peaks themselves did not resemble those seen on the example of demo chromatogram test T from the manual. I am not sure what to do or how to solve this issue. Any suggetions would be appreciated.
Do a blank run with the pump off. Do anaother with the lamp off.

My guess is that you have a chromatographic issue, not an integrator issue.
Thanks for your help. So, when I ran the blank with the pump off, the chromatogram just showed a lot of noise. When I ran the blank with the detector off, the chromatogram showed a nice straight baseline.

Also, just to clarify, what do you mean by "chromatographic issue?"
Thanks for your help. So, when I ran the blank with the pump off, the chromatogram just showed a lot of noise. When I ran the blank with the detector off, the chromatogram showed a nice straight baseline.

Also, just to clarify, what do you mean by "chromatographic issue?"
From your update, seems like an issue with the detector/lamp. A chromatographic issue would be something with the detector, the pump, the degasser, solvent quality, etc.
4 posts Page 1 of 1

Who is online

In total there are 39 users online :: 0 registered, 0 hidden and 39 guests (based on users active over the past 5 minutes)
Most users ever online was 4374 on Fri Oct 03, 2025 12:41 am

Users browsing this forum: No registered users and 39 guests

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry