-
- Posts: 3
- Joined: Wed May 25, 2011 1:06 pm
Advertisement
HP3396 integrator trouble
Discussions about chromatography data systems, LIMS, controllers, computer issues and related topics.
4 posts
Page 1 of 1
I have been working with an HP3396 Series II Integrator. It was working fine but recently started giving me trouble. I am seeing excessive noise or drift on the chromatogram when I should be seeing a baseline. I ran the tests as stated in the manual and it has passed them all. I know the power cable is fine because I swapped it for another and it works just as well. I also ran the T test and though the retention times of the peaks were the same, the peaks themselves did not resemble those seen on the example of demo chromatogram test T from the manual. I am not sure what to do or how to solve this issue. Any suggetions would be appreciated.
-
- Posts: 3594
- Joined: Mon Aug 30, 2004 11:43 pm
Do a blank run with the pump off. Do anaother with the lamp off.
My guess is that you have a chromatographic issue, not an integrator issue.
My guess is that you have a chromatographic issue, not an integrator issue.
-
- Posts: 3
- Joined: Wed May 25, 2011 1:06 pm
Thanks for your help. So, when I ran the blank with the pump off, the chromatogram just showed a lot of noise. When I ran the blank with the detector off, the chromatogram showed a nice straight baseline.
Also, just to clarify, what do you mean by "chromatographic issue?"
Also, just to clarify, what do you mean by "chromatographic issue?"
-
- Posts: 3594
- Joined: Mon Aug 30, 2004 11:43 pm
From your update, seems like an issue with the detector/lamp. A chromatographic issue would be something with the detector, the pump, the degasser, solvent quality, etc.Thanks for your help. So, when I ran the blank with the pump off, the chromatogram just showed a lot of noise. When I ran the blank with the detector off, the chromatogram showed a nice straight baseline.
Also, just to clarify, what do you mean by "chromatographic issue?"
4 posts
Page 1 of 1
Who is online
In total there are 43 users online :: 0 registered, 0 hidden and 43 guests (based on users active over the past 5 minutes)
Most users ever online was 4374 on Fri Oct 03, 2025 12:41 am
Users browsing this forum: No registered users and 43 guests
Most users ever online was 4374 on Fri Oct 03, 2025 12:41 am
Users browsing this forum: No registered users and 43 guests
Latest Blog Posts from Separation Science
Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.
Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.
- Follow us on Twitter: @Sep_Science
- Follow us on Linkedin: Separation Science