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- Posts: 20
- Joined: Thu Sep 17, 2009 9:55 pm
I am going to work on vitamin E ( α-Tocopherol, g-Tocopherol) analysis by RP-HPLC with UV detector.
Svereal papers states that vitamin E working standards are prepared and corrected daily according to their absorbance values E (1 cm/1%) α-tocopherol 75.8 at 292 nm , γ-tocopherol 92.8 at 298 nm, δ-tocopherol 91.2 at 298 nm.
Why? Is this necessary? Do we have to correct it daily?
Thank you
Svereal papers states that vitamin E working standards are prepared and corrected daily according to their absorbance values E (1 cm/1%) α-tocopherol 75.8 at 292 nm , γ-tocopherol 92.8 at 298 nm, δ-tocopherol 91.2 at 298 nm.
Why? Is this necessary? Do we have to correct it daily?
Thank you
