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Fluorescence- unit

http://www.chromforum.org/viewtopic.php?t=16475

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Fluorescence- unit

Posted: Tue May 03, 2011 6:24 pm
by Sarah1233
Hi guys

Could you please help me with this basic question.

I know that fluorescence is unitless but my detector measures it as emission or voltage units can you please explain where did those come from?

Regards
Sarah

Re: Fluorescence- unit

Posted: Wed May 04, 2011 12:05 am
by tom jupille
You always have to measure *something* :wink: . In this case it's the voltage coming from the photodetector (which is proportional to the intensity of the light hitting it.).

Re: Fluorescence- unit

Posted: Fri May 06, 2011 3:07 pm
by grumbledook
this may help http://en.wikipedia.org/wiki/Photomultiplier
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