by
DSP007 » Mon Apr 25, 2011 8:35 am
Well . Its column cromatography. This variant of licvid column chromatography use in 1950- 60 years , but now it variant use as Flash chromatography for sample concentration.
For hand make.
1) Any glass tube . You may be have n't special column, but pipette and glass wool can be make .
2) Sorbent - need use silicagel fraction 50 -100 mkm (0.05 -0.1 mm) .
Use aluminium oxide , silicagel or RP - silicagel. Silicagal have small money.
3) Problem- effective of this column is small (big granules of sorbent). Need know very effective system and light separated couples
substancies in mixture for this experiment
4) Mobile phase -you must remember that all chemicals are dangerous and protect your health. As the mobile phase, the least dangerous aqueous alcohol wipe, the type of vodka.